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Different surface glycoprotein patterns on human T‐, B‐ and leukemic‐lymphocytes
Author(s) -
Andersson Lief C.,
Wasastjerna Curt,
Gahmberg Carl G.
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910170107
Subject(s) - glycoprotein , galactose oxidase , chronic lymphocytic leukemia , leukemia , microbiology and biotechnology , polyacrylamide gel electrophoresis , biology , antigen , lymphocyte , gel electrophoresis , chemistry , biochemistry , galactose , immunology , enzyme
We have labeled the exposed surface glycoprotein of human blood T‐ and B‐lymphocytes and cells from patients with chronic lymphocytic leukemia by the galactose oxidase‐tritiated sodium borohydride method. The labeled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography. The T‐ and B‐lymphocytes show different and characteristic surface glycoprotein patterns. The surface glcoprotein patterns of the leukemic cells differ from those of normal, non‐malignant lymphocytes. A relationship between the altered surface glycoprotein pattern of leukemic cells and the expression of leumemiaassociated antigens is discussed.