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Detection of virus‐specific DNA and RNA base‐sequences in individual cells transformed or infected by adenovirus type 2
Author(s) -
Moar M. H.,
Jones K. W.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160613
Subject(s) - biology , rna , dna , adenoviridae , microbiology and biotechnology , virology , nucleic acid thermodynamics , virus , in situ hybridization , adenovirus infection , mastadenovirus , gene , messenger rna , genetics , genetic enhancement
By means of 3 H thymidine‐labelled adenovirus 2 DNA, adenovirus‐specific DNA sequences have been localized in individual nuclei of adenovirus 2‐ or 12‐infected cells, and adenovirus‐specific RNA sequences have been detected in the permissive cells as well as in cells transformed by adenovirus type 2. The ability to detect such specific virus RNA base‐sequences in particular, by in situ hybridization, should be useful in studying the transcriptional specificities of tumours and neoplasms.