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Isolation of tumor‐specific antibodies from effusions of ovarian carcinomas
Author(s) -
Dorsett Brent H.,
Ioachim Harry L.,
Stolbach Leo,
Walker John,
Barber Hugh R. K.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160510
Subject(s) - isolation (microbiology) , ovarian carcinomas , antibody , pathology , medicine , biology , ovarian carcinoma , ovarian cancer , cancer research , immunology , cancer , bioinformatics
Peritoneal effusions of patients with ovarian cancer contain sizable amounts of free and complexed immunoglobulins. By means of salt precipitation procedures, antibodies were recovered that after purification and concentration displayed a high degree of specificity against ovarian carcinoma cells. In indirect immunofluorescence, immunoglobulins recovered from seven different peritoneal effusions showed bright cytoplasmic staining with tissue cultures and fresh suspensions of ovarian carcinoma cells but not of normal ovaries or non‐ovarian tumors. Immunoglobulins isolated from fluids of benign ovarian cysts or from effusions of non‐ovarian tumors were negative in immunofluorescence tests. Autologous antibodies recovered from peritoneal effusions will be hopefully utilized in sensitive radioimmunoassay tests that are greatly needed for the early detection of ovarian cancer, the leading cause of death from gynecologic neoplasia.