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Isozyme pattern of fructose diphosphate aldolase during hepatocarcinogenesis induced by 2‐acetylaminofluorene in rat liver
Author(s) -
Silber Danuta,
Checinska Ewa,
Rabczynski Jerzy,
Kochman Marian
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160418
Subject(s) - aldolase a , 2 acetylaminofluorene , aldolase b , isozyme , fructose , carcinogenesis , biochemistry , fructose bisphosphate aldolase , carcinogen , biology , chemistry , enzyme , microbiology and biotechnology , gene , microsome
The biosynthesis of aldolase A and B subunits has been studied in rat liver during the administration of carcinogen AAF The abbreviations used in this paper are: FDP — fructose‐1,6‐diphosphate; F‐1‐P — fructose‐1‐phosphate; AAF — 2‐acetylaminofluorenea . Transition from a predominance of aldolase B to A was observed during carcinogenesis in rat liver. Changes in isozymic pattern and FDP to F‐1‐P cleavage activity ratio were observed before histological alterations typical of hepatoma could be detected. Our data support the hypothesis of dedifferentiation during hepatocarcinogenesis which in an early stage results in switching on of the gene for aldolase A with simultaneous continuation of biosynthesis of aldolase B within single cells.