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Rejection of tumor cells in vitro: A T‐cell‐mediated reaction
Author(s) -
Berczi I.,
Sehon A. H.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160417
Subject(s) - cytotoxic t cell , lymphotoxin , lymphoblast , in vitro , spleen , bone marrow , lymph , guinea pig , biology , immunology , microbiology and biotechnology , antiserum , methylcholanthrene , chemistry , pathology , cell culture , medicine , antigen , endocrinology , biochemistry , immune system , carcinogen , genetics
Cytotoxic lymphoblasts (LBC) were produced by the co‐cultivation of guinea‐pig lymphoid cells from the thymus, lymph nodes, spleen, bone marrow and blood with syngeneic methylcholanthrene‐induced sarcoma (MC‐D) cells. The cytotoxic reaction was inhibited by pretreatment of the lymphoid cell populations with a rabbit antiserum specific for guinea‐pig thymus‐derived (T) lymphocytes. Supernatants of LBC inhibited the migration of peritoneal exudate cells, but did not contain lymphotoxin or a skin reactive factor. It is concluded that the cytotoxic cells are of T‐cell origin.