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The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells
Author(s) -
Jones Peter A.,
Rhim Johng S.,
Isaacs Hart,
McAllister Robert M.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160411
Subject(s) - cell culture , osteosarcoma , hamster , extracellular , biology , in vitro , sarcoma , microbiology and biotechnology , cancer research , pathology , biochemistry , medicine , genetics
Abstract The TE‐85 osteosarcoma cell line has several of the in vitro properties of malignant cells, including colony formation in agar, but has low extracellular fibrinolytic activity and no capacity to form tumors in ATS‐treated hamsters. Some TE‐85 cell clones (clones 2, 4 and 6) have increased fibrinolytic activity but do not form tumors in hamsters. TE‐85 cells infected with mammalian transformation‐defective viruses have low (FeLV) or increased (RD‐114 virus) levels of fibrinolytic activity and do not form tumors in hamsters. TE‐85 cells either nonproductively infected with Ki‐MSV or productively infected with M‐MSV (RD‐114), have fibrinolytic activity and can form tumors in hamsters. The MSV gene(s) but not colony formation in agar or extracellular fibrinolytic activity appears to be capable of rendering TE‐85 cells tumorigenic in ATS‐treated hamsters.