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Human endometrial carcinomas serially transplanted in nude mice and established in continuous cell lines
Author(s) -
Merenda C.,
Sordat B.,
Mach J. P.,
Carrel S.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160406
Subject(s) - in vivo , transplantation , in vitro , endometrial cancer , medroxyprogesterone acetate , nude mouse , cell culture , endometrium , pathology , cancer research , biology , histology , carcinoma , cell , cancer , medicine , estrogen , endocrinology , biochemistry , genetics , microbiology and biotechnology
Three out of five human endometrial carcinomas were successfully grafted into nude mice (BALB/c/nu/nu). Two of these tumors could be maintained by serial transplantation. The morphological characteristics displayed by the grafted tumors were comparable to those of the original carcinomas. Permanent cell lines were established from these two tumors. Reinjection of cells grown in vitro into nude mice produced nodules of identical histology as compared to original solid transplants. The influence of medroxy‐progesterone acetate on tumor growth in vivo and cell proliferation in vitro was studied. This hormonal treatment did not produce any significant effect on tumor cells, either in vitro or in vivo, for the two endometrial carcinomas. After medroxyprogesterone administration, a slight but non‐significant growth inhibition of the tumor cells in vitro was observed and the tumor transplants in vivo did not appear to be influenced. The experiments illustrate the possible use of this model for testing potential anti‐cancer agents.