Premium
DNA repair synthesis of cultured human cells as a rapid bioassay for chemical carcinogens
Author(s) -
San R. H. C.,
Stich H. F.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160211
Subject(s) - carcinogen , sterigmatocystin , dna repair , aflatoxin , dna , dna damage , bioassay , biology , dna synthesis , chemistry , biochemistry , toxicology , microbiology and biotechnology , genetics
The feasibility of detection of carcinogenic chemicals using DNA repair synthesis of cultured human fibroblasts as measured by an unscheduled 3 HTdR incorporation has been explored. Of 64 chemicals tested, 29 were proximate or ultimate carcinogens, 15 were precarcinogens that required metabolic activation, 16 were non‐oncogenic compounds and 4 were of unknown carcinogenicity. All directly acting carcinogens triggered a DNA repair synthesis, whereas no unscheduled 3 HTdR incorporation was observed following the application of the 16 non‐oncogenic compounds. As a rule, the precarcinogens (without metabolic activation) do not elicit DNA repair synthesis. However, longer exposures and higher concentrations of the precarcinogens 2‐AAF, aflatoxin B 1 and sterigmatocystin gave unscheduled 3 HTdR uptake. The results suggest the suitability of using repair synthesis as endpoint, and cultured human cells as subjects in a prescreening programme for chemical carcinogens.