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The response of Xeroderma pigmentosum cells and controls to the activated mycotoxins, aflatoxins and sterigmatocystin
Author(s) -
Stich H. F.,
Laishes B. A.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160209
Subject(s) - sterigmatocystin , xeroderma pigmentosum , aflatoxin , mycotoxin , biology , dna , dna repair , dna damage , microbiology and biotechnology , genetics , food science
The activation of the mycotoxins aflatoxin B 1 , G 1 , B 2 , G 2 , aflatoxicol and sterigmatocystin by 9S fraction, microsomal preparation (105,000 × g) and supernatant (105,000 × g) of livers of several species was examined. DNA repair synthesis, chromosome aberrations and clone forming capacity were used as endpoints. Cultured fibroblasts of normal persons and DNA repair deficient Xeroderma pigmentosum patients were employed as test subjects. The activation mixtures significantly increase the chromosome breaking function, lethality and DNA damaging effect (measured as DNA repair synthesis) of aflatoxin B 1 , G 1 , aflatoxicol and sterigmatocystin. The DNA repair‐deficient XP cells respond to the activated mycotoxins with a low level of unscheduled 3 HTdR incorporation as compared to that of control cells, but show a highly elevated sensitivity to the chromosome‐damaging and lethal effect of aflatoxin B 1 and sterigmatocystin.