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Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. I. Distribution of reactivity and specificity
Author(s) -
Herberman Ronald B.,
Nunn Myrthel E.,
Lavrin David H.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160204
Subject(s) - cytotoxic t cell , spleen , cytotoxicity , biology , lymph node , neoplasm , antigen , immunology , lymphatic system , microbiology and biotechnology , in vitro , biochemistry , genetics
Lymphoid cells from many normal mice of a variety of inbred strains were found to have reactivity, in a 51 Cr release cytotoxicity assay, against several syngeneic and allogeneic tumors. Very high reactivity was seen with effector cells from athymic nude mice, which was consistent with other evidence that the reactivity was not T‐cell dependent. Target cells susceptible to lysis included tumors induced by oncogenic type‐C viruses but also tumors induced by other means and expressing endogenous type‐C viruses. The levels of natural reactivity were influenced by age, with highest cytotoxicity produced by cells from 5‐ to 8‐week‐old mice. Lymph‐node cells, spleen cells, peritoneal exudate cells and peripheral blood lymphocytes all had cytotoxic reactivity. The specificity of the reactions was analyzed in detail by an inhibition assay. Evidence was obtained for natural reactivity against several different antigens, each apparently associated with expression of murine endogenous type‐C viruses.