Premium
Immunization against Marek's disease using Marek's disease virus‐specific antigens free from infectious virus
Author(s) -
Lesnik F.,
Ross L. J. N.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160117
Subject(s) - marek's disease , virology , antigen , inoculation , virus , adjuvant , biology , immunization , antibody , immunity , immune system , microbiology and biotechnology , immunology
Immunity against Marek's disease was conferred by the use of non‐infectious materials extracted with non‐ionic detergents from cells infected with the attenuated strain of Marek's disease virus (MDV). Antibody‐free Rhode Island Red chicks were inoculated at 1 week of age with cell extracts emulsified in Freund's complete adjuvant and were given a second inoculation 1 week later without adjuvant. Protection against natural infection was obtained in groups inoculated with both soluble (not sedimented at 100,000xg/2 h) and insoluble antigens present in Nonidet P40 (NP40) extract, but only with the insoluble fraction of deoxycholate extract. The results suggest that the immunizing antigens can be partially solubilized with 0.5% NP40 and that the growth and spread of MDV are reduced in immunized chickens.