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Human tumor—lymphocyte interaction in vitro : Blastogenesis correlated to detectable immunoglobulin in the biopsy
Author(s) -
Vánky F.,
Trempe G.,
Klein E.,
Stjernswärd J.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160113
Subject(s) - antibody , lymphocyte , in vitro , immunoglobulin g , biopsy , immunoglobulin fc fragments , microbiology and biotechnology , biology , immunology , cell , lymphatic system , stimulation , pathology , reagent , chemistry , medicine , biochemistry , endocrinology
Binding of radioiodinated anti‐immunoglobulin (Ig) reagent assayed by acid elutable radioactivity was shown in 18 of 44 cell suspensions (41%) prepared from surgical specimens of human tumors. Aliquots of these biopsies were admixed to autologous lymphocytes and in 13 cases they induced stimulation of DNA synthesis. In only one of these 13 cases was the anti‐Ig reagent bound, while among the 26 biopsies with low or no binding capacity 12 (46%) were stimulatory, indicating that immunoglobulin‐containing biopsies are not stimulatory. Experiments on 32 lymphocyte preparations from different lymphoid organs suggest that the immunoglobulin detected in the tumor‐cell suspension is not derived from the infiltrating lymphoid cells.