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Spleen‐cell cytotoxicity for cytomegalovirustransformed cells. II. Inhibition by cytomegalovirus antiserum
Author(s) -
Murasko Donna M.,
Lausch Robert N.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910160105
Subject(s) - cytotoxic t cell , antigen , spleen , antibody , cytomegalovirus , cytotoxicity , microbiology and biotechnology , biology , antiserum , virology , virus , herpes simplex virus , hamster , cell , immunology , chemistry , herpesviridae , in vitro , viral disease , biochemistry
Serum from hamsters immunized repeatedly with cytomegalovirus (CMV) was able to block spleen‐cell cytotoxicity for CMV‐infected and CMV‐transformed cells. Inhibition was observed regardless of whether spleen cells were obtained from hamsters sensitized to the virus or to isografts of the transformed cells (designated Cx‐90‐3B, T‐2). Such serum did not significantly block effector cell response against transformed cells expressing herpes simplex virus or SV40‐associated membrane antigens. The mechanism of blocking was investigated. It was found that the blocking activity could be absorbed out with Cx‐90‐3B, T‐2 cells but not by untransformed hamster embryo fibroblasts. Cx‐90‐3B, T‐2 target cells treated with serum, and then washed, remained resistant to effector cell attack. On the other hand, if serum‐treated attacker cells were washed their cytotoxic activity was not significantly impaired. These results suggest that the blocking factor in the serum is antibody‐directed against cytomegalovirus‐related membrane antigen. This conclusion is supported by the finding that the serum contained antibody specifically reactive with the transformed cell surface in isotopic antiglobulin tests.