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Murine sarcoma virus related nucleic acid sequences in a non‐transforming virus derived from an interspecies pseudotype sarcoma virus
Author(s) -
Okabe Hiromi,
Gilden Raymond V.,
Hatanaka Masakazu
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150517
Subject(s) - biology , virus , virology , viral transformation , hamster , nucleic acid , helper virus , sarcoma , microbiology and biotechnology , viral replication , genetics , medicine , pathology
Abstract A number of sarcomagenic viruses recovered from hamster tumors induced originally by Moloney sarcoma virus or several of its mouse pseudotypes appear to be interspecies pseudotypes with structural proteins of hamster type‐C viruses and nucleic acid sequences of both mouse and hamster specificity. A non‐transforming virus (GLOH − ) recovered from a sarcomagenic virus preparation obtained from a tumor induced by the Gross pseudotype of Moloney sarcoma virus was previously shown to retain mouse sequences. The ability to discriminate readily between Gross and Moloney viruses by molecular hybridization, especially with regard to thermal stability of inter‐strain hybrid molecules, and the stability of Moloney virus sequences in a rat‐mouse interspecies pseudotype, made it possible to specify that the mouse virus‐specific sequences in GLOH − are derived from Moloney virus and not Gross. Thus, even though GLOH − is non‐transforming in vitro or in vivo, it still retains sequences highly related to Moloney sarcoma virus. The lack of transforming ability is thus probably based on loss or change of specific sequences necessary for transformation at some point in the history of this virus.