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Specific anti‐tumor responses by cultured immune spleen cells. I. In vitro culture method and initial characterization of factors which block immune cell‐mediated cytotoxicity in vitro
Author(s) -
Nelson Karen,
Pollack Sylvia B.,
Hellström Karl Erik
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150512
Subject(s) - spleen , cytotoxicity , in vitro , antiserum , microbiology and biotechnology , immune system , biology , cell culture , lysis , antibody , immunology , biochemistry , genetics
Spleen cells from BALB/c mice which either bore syngeneic sarcomas or were normal controls were cultured in vitro . The culture supernatants of spleen cells from tumor‐bearing mice inhibited (blocked) specific cell‐mediated cytotoxicity to the tumor borne by the spleen donor. They also mediated specific antiserum‐dependent cytotoxicity with control lymphoid cells. The appearance of blocking activity in culture supernatants was prevented by lysis of Q‐positive spleen cells with antiserum and complement. The blocking activity was removed by passing culture supernatants through an anti‐mouse immunoglobulin affinity column and was recovered in a 3 M NaSCN eluate of the column.