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Oncogenic transformation of primary hamster cells by herpes simplex virus type 2 (HSV‐2) and an HSV‐2 temperature‐sensitive mutant
Author(s) -
Kimura Susumu,
Flannery Virginia L.,
Levy Barnet,
Schaffer Priscilla A.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150510
Subject(s) - hamster , herpes simplex virus , immunofluorescence , virology , biology , cell culture , virus , antigen , microbiology and biotechnology , antibody , herpesviridae , immunology , viral disease , genetics
Six oncogenically transformed cell lines were obtained following infection of hamster embryo fibroblasts with UV‐irradiated herpes simplex virus type 2 (HSV‐2) or with an HSV‐2 temperature‐sensitive mutant. All lines produced undifferentiated fibrosarcomas in newborn hamsters, four of the six lines produced metastatic tumors in the lung, and sera from tumor‐bearing hamsters contained neutralizing antibody to HSV‐2. HSV‐specific cell‐surface antigen (s) was detected by immunofluorescence tests in all six of the lines established from primary tumors. Antibody to an early, HSV‐specific, non‐structural polypeptide (VP134) reacted by immunofluorescence with antigen (s) in fixed preparation of three of the six tumor‐cell lines.