Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas | Zendy

Lieber Michael | Zendy; Mazzetta Joann | Zendy; NelsonRees Walter | Zendy; Kaplan Michael | Zendy; Todaro George | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas

Author(s) -

Lieber Michael,

Mazzetta Joann,

NelsonRees Walter,

Kaplan Michael,

Todaro George

Publication year - 1975

Publication title -

international journal of cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.475

H-Index - 234

eISSN - 1097-0215

pISSN - 0020-7136

DOI - 10.1002/ijc.2910150505

Subject(s) - doubling time , cell culture , pancreas , carcinoma , biology , pancreatic carcinoma , epithelioid cell , cancer research , medicine , pathology , endocrinology , cancer , pancreatic cancer , immunology , immunohistochemistry , genetics

An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line was verified by: (1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast monolayer; and (2) the formation of a progressively growing anaplastic carcinoma after injection of a nude‐athymic mouse with PANC‐1 cells.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research