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Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas
Author(s) -
Lieber Michael,
Mazzetta Joann,
NelsonRees Walter,
Kaplan Michael,
Todaro George
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150505
Subject(s) - doubling time , cell culture , pancreas , carcinoma , biology , pancreatic carcinoma , epithelioid cell , cancer research , medicine , pathology , endocrinology , cancer , pancreatic cancer , immunology , immunohistochemistry , genetics
An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line was verified by: (1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast monolayer; and (2) the formation of a progressively growing anaplastic carcinoma after injection of a nude‐athymic mouse with PANC‐1 cells.