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Studies of epstein‐barr virus (ebv)‐associated nuclear antigen. I. Assay in human lymphoblastoid cell lines by direct and indirect determination of 125 i‐igg binding
Author(s) -
Brown T. D. K.,
Ernberg I.,
Klein G.
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150410
Subject(s) - raji cell , lymphoblast , antigen , epstein–barr virus , microbiology and biotechnology , virology , cell culture , biology , virus , antibody , chemistry , immunology , genetics
A quantitative assay for EBNA in human lymphoblastoid cell lines has been developed. The assay employs EBNA‐positive and ‐negative 125 I‐IgG preparations as reagents and can be used in a direct or indirect manner. EBNA specificity has been demonstrated in a number of ways. The antigenic relationship between EBNA present in Raji cells and in a number of other human lymphoblastoid cell lines of diverse origins and between the cell‐associated antigen present in FT Raji cells and that present in isolated Raji nuclei has been studied. The possibility of carrying out blocking titrations for anti‐EBNA determination has been demonstrated and the effect of glutaraldehyde and formaldehyde upon antigenic activity in FT Raji cells has been studied.