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Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations
Author(s) -
Jondal Mikael,
Pross Hugh
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150409
Subject(s) - cytotoxicity , cytotoxic t cell , lymphocyte , microbiology and biotechnology , in vitro , biology , immunology , t lymphocyte , receptor , lectin , cell culture , cell , antigen , biochemistry , genetics
The spontaneous lymphocyte‐mediated cytotoxicity (SLMC) of cells from normal donors against 19 different established cell lines was analysed. All normal lymphocytes were cytotoxic in all combinations tested in a 17 h 51 Cr release assay. SLMC was found to be mediated by a minor subpopulation of lymphocytes with Fc/C3 receptors. Noncytotoxic SRBC binding T‐lymphocytes could be induced to become cytotoxic by the addition of Con A to the incubation medium. SLMC and lectin‐induced cytotoxicity were briefly characterized. It is argued that SLMC is a non‐specific non‐immunological reaction which must be taken into consideration when lymphocytes from cancer patients are tested against tumour‐cell lines in vitro. Furthermore, SLMC and lectin‐induced cytotoxicity are proposed as functional markers for Fc/C3‐binding lymphocytes and SRBC binding lymphocytes respectively.