Premium
Distinction between tumor‐specific transplantation antigen and virion antigens in solubilized products from membranes of virus‐induced leukemic cells
Author(s) -
Chang Kenneth S. S.,
Law Lloyd W.,
Appella Ettore
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150314
Subject(s) - antigen , immunogen , antiserum , virus , complement fixation test , antibody , viral envelope , virology , biology , microbiology and biotechnology , chemistry , sephadex , pan t antigens , lysis , in vitro , cytotoxic t cell , biochemistry , immunology , serology , enzyme , monoclonal antibody
A membrane antigen from RBL‐5 leukemic cells that was solubilized and partially purified is further characterized in this study. This soluble antigen is capable of immunizing syngeneic hosts to reject neoplastic cells and thus resembles TSTA. It also induces cytotoxic antibody in syngeneic hosts capable of specifically lysing RBL‐5 cells in vitro. RBL‐5, however, releases infectious virus (RLV); it was necessary therefore to rule out virus or structural virion antigens as the effective immunogen. Infectious virus was not detectable in our initial crude membrane (CM) material, nor in the papain‐solubilized CS or the G‐150 Sephadex‐chromatographed fraction. Virus‐neutralizing antibody was not detected, under stringent assay conditions, in the syngeneic anti‐CM sera. Antigen preparations CM, CS and the chromatographed fractions F1, F2 and F3 were assayed in a complement‐fixation test against broad‐reacting antisera capable of detecting virus envelope antigen and gs antigen and against syngeneic antisera. Although our antigen preparations were positive for virion antigens. CS and F2 contained an antigen that reacted only with syngeneic antiserum. These same fractions were those reactive as immunogens. On the basis of these data, it is postulated that a cellular membrane component, other than viral, functions as TSTA.