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The influence of different isolation procedures and the use of target cells from melanoma cell lines and short‐term cultures on the non‐specific cytotoxic effects of lymphocytes from healthy donors
Author(s) -
De Vries Jan E.,
Meyering Mary,
Van Dongen Anneke,
Rümke Philip
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150305
Subject(s) - cytotoxic t cell , melanoma , cell culture , immunology , cancer research , biology , cytotoxicity , medicine , in vitro , genetics
In parallel studies the effects of FHL 1 and PNL on plated melanoma cells from cell lines and short‐term cultures were compared. FHL showed more frequent and also stronger cytotoxic and/or growth‐inhibiting effects than PNL. On melanoma target cells from cell lines both FHL and PNL showed more frequent and stronger cytotoxic and/or growth‐inhibiting effects than on melanoma target cells from short‐term cultures. In the individual donors the percentage of monocytes and EAC‐rosette‐forming cells in FHL was significantly higher than in PNL. A significant correlation was found between multiplication of the melanoma target cells during the test period and an increased susceptibility towards lymphocytes from healthy donors. Melanoma target cells from cell lines were not more fragile, or more susceptible to unfavourable culture conditions than cells from short‐term cultures, since non‐lymphocytic „effector” cells showed much weaker cytotoxic and/or growth‐inhibiting effects than lymphocytes from healthy donors. Cytotoxic effects of lymphocytes from healthy donors were also registered on target cells from a mammary carcinoma and an osteosarcoma cell line. No significant differences in the cytotoxic effects of lymphocytes from healthy donors were observed when tested on mycoplasma‐contaminated melanoma cells and the same cells made mycoplasma‐free. Mitomycin‐C‐treated lymphocytes retained their cytotoxic effects. Lymphocytes from a healthy donor tested on different occasions on the same melanoma cells from a short term culture showed an incidental cytotoxic reaction.