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Characteristics of cell lines derived from human leukocytes transformed by different strains of epstein‐barr virus
Author(s) -
Katsuki Takato,
Hinuma Yorio
Publication year - 1975
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910150205
Subject(s) - virus , epstein–barr virus , biology , virology , cell culture , antigen , in vitro , genome , microbiology and biotechnology , gene , genetics
Variation of Epstein‐Barr virus (EBV) in respect to its effect on the properties of transformed cells was probed. Human umbilical cord leukocytes from six different individuals were transformed in vitro by either B95‐8 (B) or QIMR‐WIL (Q) strains of EBV and subsequently 12 lymphoblastoid cell lines (six B‐derived and six Q‐derived lines) were established. The B lines and Q lines were different in the expression of EBV genome i.e. production of virus or viral antigens, and in other properties including growth pattern and immunoglobulin production. The most striking differences between the two groups lay in their capacity to produce infectious virus and in the shape of the cell‐clumps. The results suggest that different strains of EBV may induce transformed cells with different characteristics.