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Evaluation of epstein‐barr virus‐associated nuclear antigen with various human cell lines
Author(s) -
Suzuki Masahiro,
Hinuma Yorio
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140609
Subject(s) - virology , antigen , virus , human cell , cell culture , biology , epstein–barr virus , immunology , genetics
Critical evaluation of the anti‐complement immunofluorescence (ACIF) test for Epstein‐Barr virus‐associated nuclear antigen (EBNA) in known EBV‐associated cell lines was carried out. The ACIF procedure, in which the cell smears were thoroughly air‐dried and fixed with carbon tetrachloride (for 15 min at room temperature), provided reproducible staining results with minimum variations. The fixed smears could be stored for at least 5 weeks at −8° C without loss of reactivity. On the basis of the above evaluation, a total of 59 human cell lines, which had unknown or uncertain association with EBV, were examined for the presence or absence of EBNA by the ACIF procedure. All of the cell lines tested could be judged positive or negative for EBNA. All of 21 lines growing as monolayer or in a mixed state of monolayer and suspension, were negative. Of 38 lines grown in suspension, 36 lymphoblastoid lines were positive but the remaining 2 lines (seemingly not lymphoblastoid) were negative.