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Effect of ethidium bromide and fractionated X‐irradiation on rauscher leukemia development
Author(s) -
Okunewick James P.,
Brozovich Barbara J.,
Kuhnert Paul M.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140508
Subject(s) - ethidium bromide , virus , in vivo , leukemia , murine leukemia virus , biology , virology , microbiology and biotechnology , cancer research , immunology , biochemistry , dna
Studies were carried out to evaluate further the in vivo effectiveness of reversetranscriptase inhibitors in the therapy of Rauscher viral leukemia. Ethidium bromide, given in multiple doses, was found to be without significant effect on the survival of mice which already had established Rauscher disease as the result of injection with virus 2 weeks earlier. However, the initiation of ethidium bromide treatment immediately after virus did result in the beneficial effect of an increase in the mean survival time of the infected animals. On the other hand, at the virus titers used, 4‐N‐benzyl‐4‐N‐demthylrifampicin showed no significant therapeutic action in either case, even though other studies had suggested that it might be effective for this purpose. A total of 1,000 R of fractionated X‐irradiation given to animals with established leukemia also resulted in increased mean survival times, but the combination of irradiation and ethidium bromide treatment of similar mice resulted in only a marginal improvement over irradiation alone. It is suggested that these reverse‐transcriptase inhibitors, though active against the free virus, may not be effective against the already transformed tumor cell.