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Flow microfluorometry analysis of DNA content in chinese hamster cells following infection with simian virus 40
Author(s) -
Horan P. K.,
Jett J. H.,
Romero A.,
Lehman J. M.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140411
Subject(s) - chinese hamster , hamster , population , dna , virus , biology , microbiology and biotechnology , virology , flow cytometry , cell culture , genetics , medicine , environmental health
Infection of secondary Chinese hamster cells with SV40 virus induces a population of cells into consecutive S periods. These changes in DNA content were examined at various times post infection by means of flow microfluorometry (FMF) which enabled a kinetic analysis to be made. At 12 h post infection, a population of 6C cells was observed which was induced from late S or G 2 cells. By 24 h post infection, cells at 8C, 10C, 12C, 16C, and intermediate DNA contents were detected. The induction of this population of cells occurred within the first 24 h post infection. At later time intervals, there was a decrease in total number of cells with DNA contents of 8C or 16C.