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A microassay for humoral cytotoxicity demonstrating sublethal effects of antibody
Author(s) -
Cleveland P. H.,
McKhann C. F.,
Johnson K.,
Nelson S.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140316
Subject(s) - cytostasis , cytotoxic t cell , cytotoxicity , antibody , immune system , immunology , biology , incubation , antigen , incubation period , in vitro , biochemistry
Abstract A sensitive and objective microassay has been developed for detection of antibodies against tumor‐associated antigens of murine sarcoma cells, by measuring the ability of cells to incorporate 3 H‐thymidine after exposure to immune serum and complement. The cytotoxic effect detected by this method reflects cytostasis as well as cell death. This study also demonstrated that incubation periods longer than 4 h are required for the maximum cytotoxic effects of immune sera on sarcoma cells to become manifest.