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Influence of 5‐bromodeoxyuridine on the expression of avian GS antigen in mammalian rous sarcoma virus‐transformed cells
Author(s) -
Sovová V.,
Sainerová H.,
Urbánková M.,
Hloěánek I.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140215
Subject(s) - complement fixation test , immunofluorescence , antigen , bromodeoxyuridine , biology , rous sarcoma virus , cytoplasm , virology , microbiology and biotechnology , virus , antibody , immunoperoxidase , immunology , immunohistochemistry , serology , monoclonal antibody
The avian group‐specific (gs) antigen in the virogenic RSV‐transfarmed LW13‐RsKl and LW13‐RsK4 cells is detectable in the perinuclear zone of the cells only by immunofluorescence tests. After treatment of cells with 5‐bromodeoxyuridine (BUdR) it is detectable even by complement‐fixation tests and the immunofluorescence tests reveal its presence in the whole cytoplasm of tumour cells. The increased expression of gs antigen is, however, transient. Attempts to induce gs antigen in the non‐virogenic RSV‐transformed cells after BUdR treatement failed.