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Malignant transformation of hamster embryo cells in tissue culture by 7,10‐dimethyl benzo(c)acridine
Author(s) -
Markovits P.,
Coppey J.,
Papadopoulo D.,
Mazabraud A.,
HubertHabart M.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140210
Subject(s) - hamster , subculture (biology) , embryo , carcinogen , in vitro , cell culture , biology , tissue culture , acridine , transformation (genetics) , malignant transformation , golden hamster , microbiology and biotechnology , andrology , acridine orange , genetics , staining , medicine , botany , gene
During the 4th passage of hamster embryo cells originally treated with 7,10‐dimethyl benzo (c)acridine, characteristic colonies with transformed cells appeared. This compound is carcinogenic in mice, but its effects on cells in culture have not yet been studied. Each cell line derived from this subculture produces sarcomas in new‐born hamsters. An inoculum of 10,000 cells per animal produced visible tumors in an average time of 67 days. If the number of transplanted cells was increased, the tumors appeared sooner. These experiments, as well as others carried out in our laboratory using other carcinogens, demonstrate that hamster embryo cells cultivated in vitro can be successfully used for transformation studies.