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Cell relationships in transformation of human leukocytes by epstein‐barr virus
Author(s) -
Pope J. H.,
Scott W.,
Moss D. J.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910140115
Subject(s) - virus , biology , transformation (genetics) , macrophage , in vitro , population , virology , cell culture , cell , strain (injury) , medicine , gene , genetics , environmental health , anatomy
Transformation of human leukocytes by the QIMR‐ WIL strain of EB virus in vitro involves co‐operation between adherent cells (probably macrophages) and non‐adherent cells (predominantly lymphocytes). It was shown that the proliferating cells were derived from the non‐adherent population. Unlike macrophages, autochthonous fibroblasts did not enhance proliferation when cultured with non‐adherent cells, but apparently were inhibitory. A strain of allogeneic fibroblasts was not inhibitory. Inoculation of nonadherent cells with EB virus was essential for transformation, while inoculation of macrophages only was insufficient. It appears from these results that infection of non‐adherent cells with QIMR‐ WIL EB virus induces some cells to proliferate, and that the enhancing role of the macrophage does not involve infection with virus.