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Detection of Epstein‐Barr virus (EBV)‐associated nuclear antigen in human lymphoblastoid cell lines by means of an 125 I‐IgG‐binding assay
Author(s) -
Brown T. D. K.,
Ernberg I.,
Lamon E. W.,
Klein G.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130607
Subject(s) - lymphoblast , antigen , epstein–barr virus , cell culture , virology , microbiology and biotechnology , virus , biology , cell nucleus , cell , immunology , biochemistry , gene , genetics
The elution at low pH of human EBV‐positive 125 I‐IgG bound to preparations of EBV‐carrying non‐producer lymphoblastoid cell lines has been investigated. Specific binding was demonstrated. A number of approaches were used to confirm the specificity. The stoichiometry of binding and elution was studied. The sub‐cellular location of the EBV‐associated antigen (s) detected by this method was shown to be predominantly nuclear. This confirms other studies demonstrating the presence of a nuclear antigen in non‐producer lymphoblastoid cell lines carrying the EBV genome.