Incorporation de l'antigène tumoral de transplantation spécifique du SV40 (TSTA‐SV40) dans le virus de la stomatite vésiculleuse cultivé en cellules de hamster transformées par le SV40

Highly purified and inactivated VSV (VSV/TSV‐5 C1.2) grown in SV40 transformed hamster cells, TSV‐5 C1.2, induces in hamster a transplantation immunity against host‐tumor cells. Immunization with VSV/TSV‐5 C1.2 gave more than a 10‐fold protection against TSV‐5 C1.2 tumor cells. The transplantation immunity confered by the immunization is specific for tumor cells which possess SV40‐TSTA. VSV/TSV‐5 C1.2 protects hamsters against another SV40‐transformed cell line, EHSVi‐C1.1. In contrast, immunization with VSV/EHB, a purified preparation of VSV grown in spontaneously transformed hamster cells, EHB, gave no protection to TSV‐5 C1.2 cell challenge. It was concluded that the presence of SV40‐TSTA activity in VSV/TSV‐5 C1.2 virions was due to incorporation of this tumor cell antigen in the viral envelope. The existence of cell antigens in VSV/TSV‐5 C1.2 and VSV/EHB preparations was also shown in vitro by Cr 51 release assay. The anti‐VSV/TSV‐5 C1.2 rabbit serum reacted specifically with SV40‐transformed cells, TSV‐5 C1.2 and EHSVi‐C1.1.