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Incorporation de l'antigène tumoral de transplantation spécifique du SV40 (TSTA‐SV40) dans le virus de la stomatite vésiculleuse cultivé en cellules de hamster transformées par le SV40
Author(s) -
Ansel S.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130606
Subject(s) - hamster , microbiology and biotechnology , virology , antigen , transplantation , cell culture , immunization , in vitro , biology , chemistry , biochemistry , immunology , medicine , genetics , surgery
Highly purified and inactivated VSV (VSV/TSV‐5 C1.2) grown in SV40 transformed hamster cells, TSV‐5 C1.2, induces in hamster a transplantation immunity against host‐tumor cells. Immunization with VSV/TSV‐5 C1.2 gave more than a 10‐fold protection against TSV‐5 C1.2 tumor cells. The transplantation immunity confered by the immunization is specific for tumor cells which possess SV40‐TSTA. VSV/TSV‐5 C1.2 protects hamsters against another SV40‐transformed cell line, EHSVi‐C1.1. In contrast, immunization with VSV/EHB, a purified preparation of VSV grown in spontaneously transformed hamster cells, EHB, gave no protection to TSV‐5 C1.2 cell challenge. It was concluded that the presence of SV40‐TSTA activity in VSV/TSV‐5 C1.2 virions was due to incorporation of this tumor cell antigen in the viral envelope. The existence of cell antigens in VSV/TSV‐5 C1.2 and VSV/EHB preparations was also shown in vitro by Cr 51 release assay. The anti‐VSV/TSV‐5 C1.2 rabbit serum reacted specifically with SV40‐transformed cells, TSV‐5 C1.2 and EHSVi‐C1.1.