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Attempts to detect virus‐specific DNA in human tumors. II. Nucleic acid hybridizations with complementary RNA of human herpes group viruses
Author(s) -
Hausen Harald Zur,
SchulteHolthausen Heinrich,
Wolf Hans,
Dörries Kristina,
Egger Herwig
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130510
Subject(s) - biology , dna , virology , mononucleosis , nasopharyngeal carcinoma , virus , rna , herpes simplex virus , herpesviridae , nucleic acid , sarcoma , pathology , viral disease , medicine , gene , genetics , radiation therapy
DNA derived from various human malignant and non‐malignant tissues was hybridized with radioactive complementary RNA (cRNA) synthetized in vitro with the aid of E.coli‐RNA‐polymerase by using DNA of human herpes group viruses as templates. Epstein‐Barr virus (EBV)‐specific cRNA annealed significantly with DNA from nasopharyngeal carcinoma biopsies as well as with DNA preparations from leukocytes, bone marrow, lymph node and spleen of some patients with infectious mononucleosis. No significant hybridization was observed with either herpes simplex type 2 or type 1 cRNA and DNA from ten cervical carcinoma biopsies. cRNA of human cytomegalovirus and varicellazoster virus did not hybridize with DNA from Kaposi's sarcoma or DNA from heavily infiltrated spleens of patients with Hodgkin's disease. These data do not exclude a role of these herpes viruses in the etiology of cervical carcinoma, Kaposi's sarcoma and Hodgkin's disease. They show, however, that such a relationship (if it exists) must differ quantitatively to a considerable extent from the one observed with EBV in EBV‐associated tumors.