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Attempts to detect virus‐specific DNA in human tumors. I. Nucleic acid hybridizations with complementary RNA of human wart virus
Author(s) -
Hausen Harald Zur,
Meinhof Wolf,
Scheiber Wiltrud,
Bornkamm Georg W.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130509
Subject(s) - dna , papilloma , rna , nucleic acid , virology , biology , virus , microbiology and biotechnology , common warts , papillomaviridae , gene , human papillomavirus , cancer , medicine , genetics , pathology , cervical cancer
Human wart virus was isolated from plantar warts. After extraction of its DNA, component I was transcribed into radioactive complementary RNA (cRNA) with the aid of Escherichia coli RNA polymerase. The resulting cRNA annealed specifically to wart viral DNA and was used as a probe for the detection of wart viral DNA in human warts, condylomata acuminata, laryngeal papillomas and some malignant human tumors. High concentrations of hybridizing DNA were found in plantar warts. Verrucae vulgares annealed to a considerably lower extent only, indicating that very few genome equivalents were present in those papilloma cells. Some verrucae vulgares were found to be completely negative in this test. Condylomata acuminata, as well as laryngeal papillomas and all malignant tumors tested, did not hybridize with wart viral cRNA.