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S‐tropic murine type‐C viruses: Frequency of isolation from continuous cell lines, leukemia virus preparations and normal spleens
Author(s) -
Lieber Michael M.,
Sherr Charles J.,
Todaro George J.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130503
Subject(s) - biology , virology , virus , cell culture , murine leukemia virus , leukemia , weanling , immunology , genetics , endocrinology
A survey was undertaken to determine the prevalence of S‐tropic (xenotropic) murine type‐C viruses in continuous cell lines, leukemia virus preparations, and spleens of normal mice from various commonly used strains. Fifteen of 18 cell lines established from normal cells and transplantable tumors produced infectious type‐C viruses. For all cell lines examined, the viral isolates were either N‐, B‐, or NB‐tropic, while S‐tropic viruses were not detected. Two cell lines derived from normal tissues, however, had S‐tropic viruses which could be induced by treatment with 5‐bromodeoxyurdine, whereas cell lines derived from transplantable tumors were non‐inducible. Nine standard leukemia virus preparations likewise do not contain S‐tropic viruses. In contrast, S‐tropic viruses were recovered from spleens of normal weanling mice from six of 13 strains, indicating that the expression of S‐tropic virus is relatively common in disease‐free animals. The failure to find S‐tropic viruses in known preparations of leukemogenic murine type‐C viruses and their presence in tissues from normal animals suggest that S‐tropic viruses may play a normal physiologic role in mice.