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Virus‐augmented tumor transplantation antigens: Evidence for a helper antigen mechanism
Author(s) -
Boone Charles W.,
Paranjpe Meera,
Orme Thomas,
Gillette Ronald
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130414
Subject(s) - virus , vesicular stomatitis virus , antigen , fibrosarcoma , biology , transplantation , immunology , orthomyxoviridae , immunity , immune system , virology , priming (agriculture) , influenza a virus , medicine , surgery , botany , genetics , germination
While the homogenate of a transplantable SV40‐transformed fibrosarcoma of BALB/c mice was without tumor transplantation antigen (TTA) activity, a homogenate of the same tumor cells after infection with influenza virus or vesicular stomatitis virus retained TTA activity to a degree approaching that of whole cells. Ultraviolet inactivation of the virus in the homogenate did not affect its TTA activity. Strong evidence for a helper antigen mechanism was provided by two findings: (1) Mice made tolerant to influenza virus could no longer be made tumor‐immune with the virus‐infected tumor homogenate, and (2) priming mice with three weekly injections of egg‐grown influenza virus abrogated the ability of the virus‐infected tumor homogenate to produce tumor immunity when it was given during the fourth week. Although tumor‐cell homogenates would not induce tumor immunity, they would elicit a delayed hypersensitivity reaction in the foot pad. A working hypothesis that correlates all of the findings is proposed.