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Characteristics of murine C‐type viruses. IV. The virus produced by ehrlich tumor cells and its hybrids with mouse L cells
Author(s) -
Grundner Gertrud,
Fenyö Eva Maria,
Klein Eva
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130317
Subject(s) - virus , biology , virology , cell culture , antigen , 3t3 cells , infectivity , oncovirus , microbiology and biotechnology , genetics , transfection
The infectious properties of viruses produced by Ehrlich tumor cells (E), the A9 subline of mouse L cells (L), their hybrids (EL 0 and EL) and high‐malignant sublines of the low‐malignant hybrid line (EL m ) were investigated. The infectivity assays were antigen induction on JLS‐V9 cells, antigenic conversion and focus induction on S + L ‐ cells (D56 subline) and focus formation on BALB/3T3 cells. The E and L viruses, produced by the parental cells, were detected in the JLS‐V9 test but were distinguished in S + L ‐ and BALB/3T3 cells. The L virus was focus‐positive, the E virus focus‐negative (BALB/3T3). For antigenic conversion, the L virus was negative and the E virus positive (D56). The hybrid cell line, tested on several occasions after the hydridization event, produced viruses with characteristics similar to both E and L viruses, regardless of the complete or reduced chromosome numbers. The malignant sublines selected from the hybrid showed preferential loss of the A9 parent‐derived biarmed chromosomes. One of these lines produced virus (EL m ) with infective properties similar to those of the E virus. This suggests that the virus produced by a particular cell line is determined by the cell genome. Another malignant subline was found to be negative for production of infectious virus in all three indicator systems.