Premium
Further studies on sv40 permissive cell protein factor(s) allowing rescue of infectious virus and viral DNA from non‐shedder SV40‐transformed cells
Author(s) -
Lavialle Ch.,
Suarez H. G.,
Estrade S.,
Cassingena R.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130306
Subject(s) - permissive , nucleic acid , dna , biology , genome , virology , activator (genetics) , virus , cell , viral protein , gene , microbiology and biotechnology , genetics
The factor (s) responsible for rescue of the SV40 genome from transformed cells (activator) is essentially detected in nuclear extracts from permissive cells. Removal of nucleic acids from these extracts improves their activating capacity on transformed cells. By increasing the yield of infectious viral DNA, virions become frequently detectable. For a given experiment, a correlation is obtained between the protein concentration of the extract and the yield of infectious SV40 DNA. Unlike nuclear extracts from normal or SV40‐transformed permissive cells, those from normal nonpermissive cells are ineffective in rescuing the viral genome. The biological activity of the factor(s) is preserved at −70° C for at least 3 months.