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Malignant transformation in vitro of mouse fibroblasts by 7, 12‐dimethylbenz(A)anthracene and 7‐hydroxymethylbenz(A)anthracene and by their K‐region derivatives
Author(s) -
Marquardt H.,
Sodergren J. E.,
Sims P.,
Grover P. L.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130305
Subject(s) - anthracene , 7,12 dimethylbenz[a]anthracene , chemistry , in vitro , clone (java method) , transformation (genetics) , carcinogen , malignant transformation , toxicity , stereochemistry , biochemistry , organic chemistry , biology , cancer research , dna , dmba , gene , carcinogenesis
K‐region epoxides derived from 7, 12‐dimethylbenz(a)anthracene and from 7‐hydroxymethlybenz(a)anthracene were tested for their ability to induce malignant transformation of the M2 clone of fibroblasts derived from C3H mouse prostate. The parent hydrocarbons, the corresponding K‐region dihydrodiols and a phenol were also tested with these cells. The K‐region epoxides induced transformation but were somewhat less active than the hydrocarbons; the ring‐hydroxylated derivatives were inactive. In other experiments, the addition of α‐naphthoflavone was found to inhibit the formation of water‐soluble metabolites from and the toxicity of 7, 12‐dimethylbenz(a)anthracene without affecting malignant transformation. The results are discussed in relation to current theories regarding the metabolic activation of polycyclic hydrocarbons.