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Oncornavirus‐like particles in HeLa cells. II. Immunological characterization of the virus
Author(s) -
Bauer Heinz,
Daams Johan Hendrik,
Watson Kenneth F.,
Mölling Karin,
Gelderblom Hans,
Schäfer Werner
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130213
Subject(s) - hela , virus , virology , serology , antigen , biology , polyacrylamide gel electrophoresis , microbiology and biotechnology , chemistry , antibody , in vitro , biochemistry , enzyme , genetics
An oncornavirus‐like agent has been described in HeLa cells of human origin (Gelderblom et al. , 1974). The investigation of its protein constituents revealed a pattern similar to but different from that of known oncornaviruses. Six polypeptides with molecular weights (mw) between 12,000 d and 28,000 d, and two glycopolypeptides of about 60,000 d and 80,000 d mw were detected by SDS‐polyacrylamide gel electrophoresis. No serological cross‐reaction was found between the HeLa virus and B‐ and C‐type oncornaviruses. Essentially, none of two interspecies‐specific antigenic determinants described in mammalian C‐type viruses has been detected in the HeLa virus. However a distinct serological relationship was found between the HeLa virus and the Mason‐Pfizer monkey virus in that these viruses share at least two antigens in the mw range of 16,000 d and 28,000 d, respectively. These results confirm the electron microscopic data described before (Gelderblom et al. , 1974) and extend the evidence for a new group of oncornavirus‐like particles.