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Differences in the surface mobility between normal and SV40‐, polyoma‐ and adenovirus‐transformed hamster cells
Author(s) -
Huet Ch.,
Bernhard W.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130210
Subject(s) - concanavalin a , hamster , cell , peroxidase , microbiology and biotechnology , lectin , biology , cell culture , cell membrane , chemistry , biochemistry , enzyme , in vitro , genetics
Living cultures of normal hamster cells, and of hamster cells transformed by SV40, polyoma, Adenovirus 12 and Simian Adenovirus 7, were labelled in situ by 63 Ni‐Concanavalin A, as well as by Con A and WGA revealed cytochemically by peroxidase, and by negative colloidal iron. They were then incubated at 37°C in PBS for different periods of time, varying from 8 min to 4 h prior to fixation and processing for electron microscopy. Con A labelling allowed us to show differences in the surface movements between normal and virus‐transformed cell cultures: the transformed cell lines had a much greater membrane mobility since most of the Con A/peroxidase‐labelled material disappeared from the cell surface within 15 to 30 min, whereas in normal cells it took 30 to 60 min to obtain the same patterns. Measurements of radioactive Con A showed that most of the lectin penetrated into the cell, and that shedding of labelled cell coat material into the medium was minimal. With the negative colloidal iron, the available amino groups were labelled and the same difference in the surface movements was again observed. However, we were unable to show any difference between normal and transformed cells with the WGA‐peroxidase complex. Rapid penetration occurred in both cell types, but in both cases label also remained present at the surface.