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In vitro cloning of a rat ascites hepatoma cell line, AH66, with special reference to alpha‐fetoprotein synthesis
Author(s) -
Tsukada Yutaka,
Mikuni Masahiko,
Hirai Hidematsu
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130207
Subject(s) - alpha fetoprotein , ascites , in vitro , biology , cell culture , cloning (programming) , microbiology and biotechnology , chromosome , alpha (finance) , cell , biochemistry , genetics , medicine , gene , hepatocellular carcinoma , construct validity , nursing , patient satisfaction , computer science , programming language
Cell clones producing low amounts of alpha‐fetoprotein were isolated from AH66 cell line from an ascites hepatoma producing high amounts of AFP in vitro . The growth rate and the chromosome constitution were compared between clones producing high and low amounts of AFP. No significant correlation was found between the rate of AFP production and the chromosome number. A slight increase in host survival time was observed when the rats were transplanted with low‐AFP‐producing clones compared with high‐AFP‐producing clones.