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Effect on anti‐alpha‐fetoprotein serum on some cultured tumor cells
Author(s) -
Tsukada Yutaka,
Mikuni Masahiko,
Watabe Hiroyuki,
Nishi Shinzo,
Hirai Hidematsu
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130206
Subject(s) - antiserum , cytotoxic t cell , biology , microbiology and biotechnology , antigen , antibody , cell culture , population , alpha fetoprotein , cell , immunofluorescence , in vitro , immunology , biochemistry , medicine , cancer research , hepatocellular carcinoma , genetics , environmental health
The cytotoxic effect of horse anti‐α‐fetoprotein (AFP) sera on some cultivated rat tumor cells was investigated. Antisera inactivated at 56°C for 30 min revealed a selective inhibitory effect on the gowth of AFP‐producing cells of a rat ascites hepatoma line, AH66. However, a small portion of the cell population survived and gradually increased in number. These viable cells were found to be low‐AFP‐producing cells. Extremely low‐AFP‐producing cells could be separated by repeated treatment with the anti‐AFP sera. The survival time of rats transplanted with these low‐AFP‐producing cells was about twice as long as with the original AH66 cell line. The growth of high‐AFP‐producing clones of the AH66 cell line was inhibited within 48 h of cultivation. On the other hand the growth of low‐AFP‐producing clones was only slightly inhibited. The positive membrane immunofluorescence test of AFP‐producing cells suggested that anti‐AFP antibody adhered to AFP secreted on the cell surface. This antigen‐antibody binding on the cell surface may inhibit cell multiplication. The cytotoxic index and the rate of AFP production were closely correlated. Under the experimental conditions of this study, there was no observable effect when complement was added to the antisera.