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Sensitivity to guinea‐pig serum of hamster cells transformed in vitro by two strains of the rous sarcoma virus
Author(s) -
Bataillon G.
Publication year - 1974
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910130103
Subject(s) - rous sarcoma virus , hamster , baby hamster kidney cell , virology , in vitro , virus , biology , antigen , neuraminidase , microbiology and biotechnology , guinea pig , cytotoxic t cell , immunology , biochemistry , genetics
Whereas cloning efficiency in soft agarose of untransformed hamster BHK 21/13 cells is strongly reduced when the cells are pre‐incubated with guinea‐pig (GP) serum, BHK 21/13 cells transformed by the Bryan strain of Rous sarcoma virus are unaffected by the same treatment; sensitivity of BHK 21/13 cells transformed by the Schmidt‐Ruppin strain of Rous sarcoma virus is intermediate. It is suggested that GP serum contains heterospecific cytotoxic substances directed against a xenogeneic surface antigen present on BHK 21/13 cells, and to a lesser extent, on BHK 21/13 cells transformed by SR‐RSV; this antigen is not detectable on the surface of BHK 21/13 cells transformed by the B‐RSV but can be “unmasked” after treatment with neuraminidase.