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Differences in cytochalasin B inhibition of 3‐O‐methylglucose uptake between BALB/3T3 cells and a murine sarcoma virus transformed clone
Author(s) -
Graff Jon C.,
Hanson David J.,
Hatanaka Masakazu
Publication year - 1973
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910120308
Subject(s) - cytochalasin b , cytochalasin , biology , cell culture , 3t3 cells , cytochalasin d , microbiology and biotechnology , cell , biochemistry , genetics , cytoskeleton , transfection
Cytochalasin B, a mold metabolite, in concentrations of 10 μg per ml, showed a different inhibitory effect on the uptake of 3‐O‐methylglucose between a cell line transformed by the Kirsten strain of murine sarcoma virus, and its non‐transformed parent line. When the overall uptake of 3‐O‐methylglucose by the non‐transformed BALB/3T3 cells in the presence of cytochalasin B was compared to the sarcoma‐transformed cell under the same conditions, it was found that the sugar “infiltrated” into the transformed cells despite the inhibitory effect of cytochalasin B, but not into the control cells. This difference in the infiltration of 3‐O‐methylglucose in the presence of 10 μg/ml of cytochalasin B in the cells occurred despite an almost complete inhibition of the uptake of 2‐deoxyglucose in both cells caused by that same concentration of cytochalasin B. The rate of uptake of 3‐O‐methylglucose was found to be more rapid in the transformed cell line than in the parental, non‐transformed cell line. Neither the increased rate of 3‐O‐methylglucose uptake nor the “infiltration” of the sugar in the presence of cytochalasin B in the transformed cell was caused by a newly induced system for metabolizing 3‐O‐methylglucose. The 3‐O‐methylglucose uptake system was found to be a carrier‐mediated facilitated diffusion system. This system had, at least in part, a common carrier with 2‐deoxyglucose.