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Transformation of mouse cells infected with akr leukemia virus by benzene‐extract fractions of city air particles
Author(s) -
Rhim J. S.,
Gordon R. J.,
Bryan R. J.,
Huebner R. J.
Publication year - 1973
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910120219
Subject(s) - in vitro , embryo , leukemia , virus , tissue culture , biology , transformation (genetics) , benzene , microbiology and biotechnology , chemistry , virology , biochemistry , immunology , gene , genetics , organic chemistry
Fractions of benzene extract of particles from city air have been studied for their transforming activity in the AKR leukemia‐virus‐infected NIH Swiss mouse embryo (AKR‐NIH‐ME) cell system. Mouse embryo cells chronically infected with AKR leukemia virus were transformed by benzene smog extracts, most of its fractions, and certain chemicals, whereas uninfected mouse embryo cultures were not transformed. Most of the transformed cells produced tumors when transplanted into newborn NIH Swiss mice. The three neutral fractions: AE 0 S 2 (tetracyclics), AE 0 S 3 (pentacyclics, including benzo(a)pyrene) and AE 0 S 5 (unknown structural type) and two acid, phenol fractions, AE 4 and AE 4 B, had the strongest transforming activity in vitro. One fraction, AE 1 B(CHCl 3 back extract of water washes from AE 1 ) appeared to be inactive in in vitro systems at the concentrations tested.