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The deficient density‐dependent growth control of human malignant glioma cells and virus‐transformed glia‐like cells in culture
Author(s) -
Westermark Bengt
Publication year - 1973
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910120215
Subject(s) - cell culture , biology , glioma , cell growth , neoplastic cell , cell cycle , cell division , cell , dna synthesis , virus , neoplastic transformation , in vitro , microbiology and biotechnology , cancer research , immunology , carcinogenesis , genetics , cancer
Abstract The density‐dependent growth control of eight established human glioma lines and virus‐transformed human glia‐like cells has been investigated. All neoplastic lines reached higher terminal cell densities than the corresponding normal glia‐like cells. A marked decrease in proliferation rate occurred at high cell densities which could not be explained by medium depletion or accumulation of toxic substances. An absolute resting phase was not reached by the neoplastic cells; a considerable residual DNA synthesis continued even at the highest cell density levels which could be reached. The intervals of the cell cycle were determined on one glioma line. Cells inhibited in crowded cultures were arrested in G1. The results imply that at least a proportion of neoplastic human glia lines retain a degree of topoinhibition, i.e. are still sensitive to growth restraint induced by increased cell density. However, topoinhibition was never as complete as among normal cells, since possibly cell density‐independent (“autonomous”) multiplication was maintained at a fairly high rate even at the abnormally high cell densities which all neoplastic lines attained.