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Reversion in hybrids between SV40‐transformed hamster and mouse cells
Author(s) -
Wiblin C. N.,
Macpherson I.
Publication year - 1973
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910120116
Subject(s) - hamster , reversion , biology , cell culture , microbiology and biotechnology , chinese hamster , bromodeoxyuridine , virology , chromosome , cell fusion , karyotype , phenotype , genetics , cell growth , gene
The expression of the transformed phenotype was studied in segregants from a hybrid cell line resulting from the fusion of SV40‐transformed BHK21 Syrian hamster cells (thioguanine resistant) and 3T3 mouse cells (bromodeoxyuridine resistant). Since the hamster cell parent was sensitive to bromodeoxyuridine, segregants lacking chromosomes could be obtained by “back‐selection” in this drug. These segregants retained transformed characteristics, despite substantial loss of hamster chromosomes. Selected segregants were treated further with cytotoxic antibody raised against the hamster parent. The majority of the segregants isolated retained all transformed characteristics, even when the hamster chromosome complement was greatly reduced. Some derivatives showed properties intermediate between a transformed and an untransformed state. Two revertant clones were also isolated which lacked transformed characteristics and contained no metacentric (hamster) chromosomes. Unlike the other segregants, no SV40 virus could be recovered from these cells. The implications of these results with respect to the integrated status of transforming SV40 genomes are discussed.