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In vivo and in vitro binding of carbon tetrachloride with nucleic acids and proteins in rat and mouse liver
Author(s) -
Rocchi P.,
Prodi G.,
Grilli S.,
Ferreri A. M.
Publication year - 1973
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910110219
Subject(s) - carbon tetrachloride , nucleic acid , in vivo , in vitro , microsome , biochemistry , dna , methylcholanthrene , polynucleotide , rna , chemistry , microbiology and biotechnology , metabolite , biology , carcinogen , gene , organic chemistry
14 C‐labelled carbon tetrachloride binds in vivo to DNA of mouse liver and to r RNA of rat liver if the animals have been pretreated with 3‐methylcholanthrene. A noticeable amount of radioactivity is also observed in liver proteins. In vitro carbon tetrachloride is activated by microsomes and pH 5 enzymes of 3‐methylcholanthrene‐treated animals to a metabolite which can react with DNA and polynucleotides; this effect is more evident if subcellular fractions from mouse liver are used.