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Synthesis of murine leukemia virus in cell culture
Author(s) -
Okano Hiromitsu,
Rich Marvin A.,
Johns Lewis,
Rich Ruth
Publication year - 1973
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910110111
Subject(s) - rna , uridine , cytoplasm , labelling , biology , nucleolus , virus , virology , microbiology and biotechnology , murine leukemia virus , leukemia , cell culture , cell nucleus , biochemistry , genetics , gene
Using high resolution, electron microscopic autoradiography, the times for macromolecular synthesis and assembly of a murine leukemia virus were determined. The time between completion of viral, RNA and protein synthesis and the appearance of morphologically complete virions did not exceed 30 min. The sequence of cellular labeling following exposure to 3 H‐uridine was: nucleolar; nuclear; cytoplasmic. Labelling kinetics for viral RNA paralleled that for cytoplasmic RNA and coincided with a decrease in grain counts over nucleolar areas. Comparison of labelling kinetics for type‐A and ‐C virions supports the idea the type‐A virions are precursors for type‐C.