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Surface antigen and polypseudopodia in abortive transformation of BHK 21 cells by polyoma virus
Author(s) -
Stoker M. G. P.,
Thornton Maureen,
Riddle P.,
Birg Françoise,
Meyer G.
Publication year - 1972
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910100321
Subject(s) - mitosis , antigen , stimulation , virus , biology , virology , cell , immunofluorescence , microbiology and biotechnology , transformation (genetics) , cell division , dna synthesis , dna , antibody , immunology , biochemistry , gene , neuroscience
When BHK 21 cells are exposed to high multiplicities of polyoma virus, a change in surface antigen may be detected by immunofluorescence, beginning at 18 h and affecting up to 88% of cells 24 h after infection. Time‐lapse cinematography shows that there is a concurrent change in movement and shape (polypseudopodia) of the cells in the confluent layer, involving about the same proportion of cells. Both these changes appear before the virus‐induced stimulation of the thymidine incorporation and mitosis, and before the increase in lectin agglutinability. The S antigen disappears more rapidly than the polyspeudopodia in these abortively transformed cells, but both persist in stably transformed cells. The antigenic change and polyspeudopodia are not the result of the virus‐induced stimulation of cell DNA synthesis and mitosis, but it is not yet possible to say if they are an essential requirement for this stimulation.